New assay using fluorogenic substrates and immunofluorescence staining to measure cysteine cathepsin activity in live cell subpopulations
نویسندگان
چکیده
منابع مشابه
Live Cell Imaging and Profiling of Cysteine Cathepsin Activity Using a Quenched Activity-Based Probe.
Since protease activity is highly regulated by structural and environmental influences, the abundance of a protease often does not directly correlate with its activity. Because in most of the cases it is the activity of a protease that gives rise to its biological relevance, tools to report on this activity are of great value to the research community. Activity-based probes (ABPs) are small mol...
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ژورنال
عنوان ژورنال: Cytometry Part A
سال: 2007
ISSN: 1552-4922,1552-4930
DOI: 10.1002/cyto.a.20365